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. Author manuscript; available in PMC: 2021 Sep 8.
Published in final edited form as: Nat Chem Biol. 2021 Mar 8;17(5):593–600. doi: 10.1038/s41589-021-00757-y

Fig. 4 |. Multiple nanobody-tag pairs are applicable for protein-selective deglycosylation.

Fig. 4 |

a, Schematic of nanobodies against the EPEA and 14-residue Ubc tag adapted to split OGA. b, nEPEA-splitOGA or nUbc-splitOGA remove O-GlcNAc from Nup62 tagged with Ubc and EPEA. Anti-myc and anti-HA blots detect expression of N2 and nanobody-C3, respectively. Results are representative of two biological replicates. c, c-Fos with Ubc tag can be selectively deglycosylated by nUbc-splitOGA. Representative immunoblots are shown. Quantitative results are the mean ± s.d. of n = 3 independent experiments, using unpaired two-tailed Student’s t tests for statistical analysis. n.s., not significant. WCL, whole cell lysate.