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. Author manuscript; available in PMC: 2021 Sep 8.
Published in final edited form as: Nat Chem Biol. 2021 Mar 8;17(5):593–600. doi: 10.1038/s41589-021-00757-y

Extended Data Fig. 3. nGFP-OGA(GS-ΔHAT) has limited target protein selectivity and can alter subcellular localization of the target protein.

Extended Data Fig. 3

a, nGFP-OGA(GS-ΔHAT) removes O-GlcNAc from Nup62 without GFP tag similar to the full length OGA (fl-OGA). HEK 293T whole cell lysates (WCL) and immunoprecipitation samples were analyzed by immunoblotting assays using the indicated antibodies. Results are representative of two biological replicates. b, nGFP colocalizes with nuclear transcription factor Sp1 with GFP and does not change the subcellular localization of GFP-Sp1 by immunofluorescence imaging. c, nGFP-OGA(GS-ΔHAT) alters the subcellular localization of GFP-Sp1, but co-expression with fl-OGA does not change nuclear localization of GFP-Sp1 by immunofluorescence imaging. Channels are annotated on the top. Scale bar: 10 μm. Right: merged channel. Proteins co-expressed in each sample were labeled on the left side. Images are representative of at least three randomly selected frames.