KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| BRPNc82 | Developmental Studies Hybridoma Bank | RRID: AB_2314866 |
| GluRIIA | Developmental Studies Hybridoma Bank | RRID: AB_528269 |
| GluRIID | [27] | N/A |
| Spinophilin | [18] | N/A |
| Unc13A | [26] | N/A |
| Unc13B | [26] | N/A |
| RIM-BP | [26] | N/A |
| PAK | [30] | N/A |
| Goat anti-mouse Alexa-Fluor-488 | Invitrogen | Cat# A-11001; RRID: AB_2534069 |
| Goat anti-rabbit-Cy3 | Jackson ImmunoResearch | Cat# 111–165-144; RRID: AB_2338006 |
| Goat anti-guineapig-Cy3 | Jackson ImmunoResearch | Cat# 106–165-003; RRID: AB_2337423 |
| Anti-Horseradish Peroxidase Alexa-Fluor-647 | Jackson ImmunoResearch | Cat# 123–605-021; RRID: AB_2338967 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Vectashield | VectorLabs | Cat# H-1900–10 |
| ProLong™ Gold Antifade Mountant | Thermo Fisher Scientific | Cat# P36930 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: w1118 | Bloomington Drosophila Stock Center | BDSC: 5905; FlybaseID: FBst0005905 |
| D. melanogaster: spnΔ3.1 or spex3.1 | [18] | N/A |
| D. melanogaster: Df(3L)BSC116 | Bloomington Drosophila Stock Center | BDSC: 8973; FlybaseID: FBst0008973 |
| D. melanogaster: syd-1ex1.2 | [15] | N/A |
| D. melanogaster: syd-1ex3.4 | [15] | N/A |
| D. melanogaster: nrx-1241 | [23] | N/A |
| D. melanogaster: Df(3R)5C1 | Bloomington Drosophila Stock Center | BDSC: 1605; FlybaseID: FBst0001605 |
| D. melanogaster: nlg1ex1.9 | [10] | N/A |
| D. melanogaster: nlg1ex2.3 | [10] | FlybaseID: FBal0246577 |
| D. melanogaster: nlg2CL5 | [11] | FlybaseID: FBal0285173 |
| D. melanogaster: GluRIIAGFP | [30] | N/A |
| D. melanogaster: Unc13Pacman | [26] | N/A |
| D. melanogaster: Del100BPacman | [26] | N/A |
| D. melanogaster: unc-13EMS7.5 | [26] | FlybaseID: FBal0338122 |
| D. melanogaster: unc-13 P84200 | KYOTO stock center | DGRC# 101911 |
| D. melanogaster: unc-13AGoF | This paper | N/A |
| D. melanogaster: unc13BGoF | This paper | N/A |
| D. melanogaster: Ok6-Gal4 | [64] | N/A |
| D. melanogaster: Ok319-Gal4 | [65] | N/A |
| D. melanogaster: UAS-TNT-E | [35] | N/A |
| D. melanogaster: UAS-BONT-C | This paper | N/A |
| D. melanogaster: UAS-Unc13B-RNAi | [66] | N/A |
| D. melanogaster: unc13BCRISPR | This paper | N/A |
| Oligonucleotides: | ||
| GGGCGAGGAAAAAGTAGCTCTTTACCACGTACAATATACCTGTTTACACG | This paper | N/A |
| CGTGTAAACAGGTATATTGTACGTGGTAAAGAGCTACTTTTTCCTCGCCC | This paper | N/A |
| AATTGAGCTCCCACCATGCCAATAACAATTAACAAC | This paper | N/A |
| TTAAGGTACCTTATTTATTATATAATGATCTACCATC | This paper | N/A |
| AGAGCTCCGCTCTTAAGCCA[GGG] | This paper | N/A |
| Software and Algorithms | ||
| Fiji | [61] | http://fiji.sc/ |
| GraphPad Prism 5 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| GraphPad Prism 8 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Imspector Image Acquisition & Analysis Software v16.1. | Abberior Instruments | http://www.imspector.de |
| pClamp 10 | Molecular Devices | https://www.moleculardevices.com/ |