Extended Data Figure 7. The rhythmicity of SCN^GABA firing in glucose metabolism.

(a) Experimental design for chemogenetic activation of the SCN^GABA neurons in WT mice with hM3Dq. (b) Body weight of VGAT-Cre mice injected with AAV expressing hM3Dq or control mCherry, n = 11 mice. Mice were injected at the age of 2 months old. (c) Experimental design for chemogenetic repression of the SCN^GABA neurons in WT and KO mice with hM4Di. (d) Body weight of WT and KO injected with AAV expressing hM4Di, n = 12 mice for WT, n = 14 mice for KO. Mice were injected at the age of 2 months old. (e-f) GTT of WT or KO mice injected with AAV expressing hM4Di at the indicated ZT in the presence of CNO or saline, n = 12 mice for WT, n = 14 mice for KO. Data are mean ± S.E.M. * p < 0.05 by two-sided t-test.