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. Author manuscript; available in PMC: 2021 Aug 18.
Published in final edited form as: Nat Microbiol. 2021 Feb 18;6(5):658–671. doi: 10.1038/s41564-021-00866-3

Extended Data Fig. 7. SDS-PAGE analyses of the elution fractions of the size-exclusion chromatography analyses of SopD carrying mutations in amino acids defining its interface with Rab8.

Extended Data Fig. 7.

Purified Rab8a1-183 preloaded with GDP was incubated with purified SopD or the indicated SopD mutants and subjected to size-exclusion chromatography (see the chromatographic profile in Fig. 3e). Fractions were collected, separated on an SDS-PAGE, and stained with Coomassie blue. This experiment was conducted at least three times with equivalent results.