Skip to main content
. Author manuscript; available in PMC: 2021 Aug 18.
Published in final edited form as: Nat Microbiol. 2021 Feb 18;6(5):658–671. doi: 10.1038/s41564-021-00866-3

Extended Data Fig. 9. SopD lacks guanine-nucleotide exchange activity.

Extended Data Fig. 9.

Mant-GDP-loaded Rab8a (2 μM) was incubated with Rabin8 (0.4 μM) (positive control), SopD (2 μM), 2 μM SopDR312A (2 μM), SopDE293A (2 μM) or buffer only (negative control), in the presence of 5 μM GTPγS. The decreased fluorescence as a result of the mant-GDP to GTPγS exchange was monitored over time. Data are expressed as the mean ± SD from three independent experiments.