Extended Data Fig. 2. Rab8 is recruited to the Salmonella-induced membrane ruffles.

Henle-407 cells were transiently transfected with a plasmid expressing GFP-Rab8A and subsequently infected with wild-type S. Typhimurium, its ΔsopD isogeneic mutant, or left uninfected (mock). Fifteen minutes after infection, cells were fixed and stained with anti-GFP antibody, to visualize Rab8A (green), rhodamine-labeled Phalloidin, to visualize the actin cytoskeleton (red), and 4’,6-diamidino-2-phenylindole (DAPI) to visualize nuclear and bacteria DNA (blue). Scale bar: 10 μm. This experiment was conducted at least three times with equivalent results.