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. Author manuscript; available in PMC: 2022 Apr 30.
Published in final edited form as: Circ Res. 2021 Feb 19;128(9):1300–1316. doi: 10.1161/CIRCRESAHA.120.318503

Figure 8. C/EBPβ overexpression represses canonical Wnt signaling in pAVICs.

Figure 8.

(A) Western Blot to show active β-catenin expression in whole pAVIC lysates cultured in OM, and in the presence of 400ng/μl rC/EBPβ (n=2). (B) Quantitation of Western Blot shown in (A). (C) Western blot and densitometric analysis (D) of Axin1 from pAVICs transfected with pcDNA3-C/EBPβ (pC/EBPβ) or empty pcDNA3 (n=3). *P-value using corrected Mann-Whitney: 0.03. (E) Luminescence as %RLU/mg in pAVICs transfected with pC/EBPβ or empty pcDNA controls, along with Axin1-Luciferase plasmid, in presence of DMSO or 100nM KPT-330 (n=3). *P-value using corrected Mann-Whitney: empty pcDNA3+DMSO vs empty pcDNA3+KPT 0.03, empty pcDNA3+DMSO vs pC/EBPβ+DMSO 0.03, empty pcDNA3+KPT vs pC/EBPβ+KPT 0.03, pcDNA3+DMSO vs pC/EBPβ+KPT 0.2. (F) Alizarin Red images and quantification (G) of positive Alizarin Red stained area/field in pAVICs cultured in OM or OM with XAV-939 (XAV) (n=3). *P-value using corrected Mann-Whitney: 0.03. (H) Immunofluorescence staining images and quantitation (I) of pHH3 positive pAVICs cultured in OM or OM with XAV-939 (n=3). *P-value using corrected Mann-Whitney: 0.03.