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. 2021 Apr 29;11:9335. doi: 10.1038/s41598-021-88599-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Diazepam effects in the open field. (A) (Left) Cumulative distance moved in the open field. (Middle and right) Mean (± SD) distance after 5 (middle) and 10 min (right). White circles show data for individual animals. Animals were injected i.p. with vehicle or three doses of diazepam (0.5, 1.0 and 2.0 mg/kg) thirty minutes prior to behavior testing, as labeled. (B–D) As before, but for the time spent moving (B), number of entries in the center zone (C) and time spent there (D). Diazepam reduced locomotor activity and the number of entries in the center zone. *p < 0.01, ***p < 0.0001, one-way ANOVA followed by Tukey’s post hoc test.