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. Author manuscript; available in PMC: 2021 Jul 25.
Published in final edited form as: Nat Microbiol. 2021 Jan 25;6(5):584–593. doi: 10.1038/s41564-020-00853-0

Figure 1. FtsW is an essential septum specific PGTase.

Figure 1.

a. Representative images of E. coli cells of different strain backgrounds labeled with AF647-conjugated NAM in the absence or presence of MTSES from three independent experiments. The contrast of each image is adjusted for visualization of septal labeling especially for the Δ3 ponBS247C ftsWI302C and murJA29C + MTSES conditions. The absolute intensity is summarized in Supplementary Table 3. Scale bars: 1 μm. b. Mean percentage of cells with septal NAM labeling above background level in the absence or presence of MTSES. c. Mean percentage of total loss in septal NAM intensity of the five strains due to MTSES. Error bars are S.E.M. of three experimental repeats (dots). Strains used were BW25113 (wt), JXY559 (ftsWI302C), HC532 (Δ3 ponBS247C), JXY564 (Δ3 ponBS247C ftsWI302C), and JXY589 (murJA29C), all carrying plasmid pBBR1-KU.