Extended Data Fig. 2. Representative septal FtsW-RFP trajectories are segmented and fit with line to extract directional moving speeds.

Raw trajectories of single FtsW-RFP molecules along the septal circumference are demonstrated as orange lines. Gary, light-yellow, and light-blue shades indicate segments of stationary, low-moving, and fast-moving states respectively. Corresponding speeds, obtained by linear fitting, are shown as solid line and labeled in each sub-figure. Supplementary Movies 6 to 9 correspond to trajectories a to d.