Figure 1: The divisome consists of two dynamically distinct subcomplexes.

a Schematic of divisome proteins in B. subtilis, with early-arriving proteins in blue and late-arriving proteins in red. Light blue: FtsZ filament, dark blue: FtsZ binding proteins, light red: trimeric complex, dark red: cell wall synthesis enzymes. All blue proteins are stationary, and all red proteins move directionally with the same velocity. b Kymographs of single molecules of stationary ZBPs at division sites, from two replicates for each condition. c Kymographs of single molecules of directionally-moving proteins at division sites, from at least two replicates for each condition. d Velocity distributions of all directionally-moving proteins, measured from kymographs. Scale bars: horizontal: 2 μm, vertical: 1 min.