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. Author manuscript; available in PMC: 2021 Sep 18.
Published in final edited form as: Nat Microbiol. 2021 Mar 18;6(5):553–562. doi: 10.1038/s41564-021-00878-z

Extended Data Fig. 3. Effects of individual ZBP knockouts on cell and Z ring morphology.

Extended Data Fig. 3

Each pair of images shows cell morphology (phase-contrast imaging, left), and Z ring morphology (epifluorescence images of cells expressing FtsZ-mNeonGreen induced with 20 μM IPTG for 2 hours, right) in control cells, compared to cells with individual ZBPs deletions. ΔezrA cells less condensed Z rings, along with the expected Z rings near their poles33; ΔsepF and ΔzapA cells have normal Z rings. FtsZ(T111A) mutant cells have excess Z rings and form mini-cells. The distribution of Z ring widths in each strain is plotted at bottom left. Representative images from at least two replicates of each condition. Scale bars: 2 μm.