Skip to main content
. Author manuscript; available in PMC: 2021 Sep 18.
Published in final edited form as: Nat Microbiol. 2021 Mar 18;6(5):553–562. doi: 10.1038/s41564-021-00878-z

Extended Data Fig. 5. Effects of removing synthetically lethal combinations of ZBPs on cell and Z ring morphology.

Extended Data Fig. 5

Each pair of images shows cell morphology (phase-contrast imaging, left), and Z ring morphology (epifluorescence images of cells expressing FtsZ-mNeonGreen induced with 20 μM IPTG for 2 hours, right), in control cells and cells lacking synthetically lethal combinations of ZBPs. To achieve this, a combination of knockouts (indicated by Δ) and depletions (indicated by ↓) were used; depletions were performed by expressing each gene under an inducible promoter until the start of the experiment, then withdrawing the inducer for 7 hours. This was repeated for all permutations of synthetically lethal combinations of ZBPs; all of these combinations result in elongated cells and disrupted Z ring architecture. Representative images from at least two replicates of each condition. Scale bars: 2 μm.