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. 2021 Apr 29;6:65. doi: 10.1038/s41541-021-00322-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The probability of achieving the 1.5 g/L RNA effective yield CQA under 20% standard deviation in the kinetic rate constant model parameters at a fixed 10⁻⁸ M T7RNAP concentration. The probability is illustrated by the colour code. The black cross represents the cost-optimal point.