Table 2.

Key modelling input and output results.

I/O	Parameter	Unit	Value
Input	NTP concentration	mM	40.8
	T7RNAP concentrationa	M	1.5 × 10−8
	Mg concentrationb	mM	85
Output	Yield in bioreactor	g × L−1	4.34
	Cost of T7RNAP and NTPs per g of RNA	USD × g−1	2740

^aThe T7RNAP concentration range for optimisation was 0.5 × 10⁻⁸−1.5 × 10⁻⁸.

^bThe Mg concentration was the experimental optimum and it was not subject to cost-yield optimisation due to the low purchase cost of this material.