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. 2021 Apr 29;11:9341. doi: 10.1038/s41598-021-88835-y

Figure 1.

Figure 1

The effect of sequential centrifugation on plasma PPL clotting times. Citrated blood collected from 6 healthy volunteers was subjected to consecutive centrifugations: 2500×g for 15 min to obtain platelet poor plasma (PPP), 2500×g for 15 min to obtain platelet free plasma (PFP), 20,000×g for 30 min and 100,000×g for 1 h to deplete for EVs. A sample of plasma after each centrifugation was analyzed with the modified PPL assay. The PPL activity is presented in seconds (s) of clotting time. Dot plot with mean ± 1 SD (n = 6 for each condition).