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. 2021 Apr 29;11:9341. doi: 10.1038/s41598-021-88835-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of platelet free plasma (PFP) preparation on clotting time. PFP from 10 volunteers were prepared using two different centrifugation protocols, (A) 2500×g for 15 min twice or (B) 3000×g for 10 min followed by 13,500×g. Clotting times were measured using the modified PPL assay. Panel (A) is a box plot of all ten volunteers combined for the two protocols, while panel (B) displays the individual values as mean of duplicate measures ± 1 SD.