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. 2021 Apr 29;11:9341. doi: 10.1038/s41598-021-88835-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of coagulation factors on the performance of the modified PPL assay. Exogenous coagulation factors were added to the reaction buffer in concentrations from 0.02 to 3.0 nM for FVa (panel A) and from 5 to 80 nM for FVIIa (panel B). Panel (C) shows the effect of lactadherin pretreatment on the PPL activity of PRP-derived EVs generated by stimulation of PRP by calcium ionophore A23187 for 15 min. Values are means ± 1 SD (n = 4).