Table 1.
Primers used for polymerase chain reaction (PCR) amplification and sequencing of mt/apico.
| Primer sequence (5′ → 3′) | Amplified position | Amplicon | |
|---|---|---|---|
| mt | F: AAGCTTTTGGTATCTCGTAATGTAGAA R*: TTAGCAATAACATTCCTGATGTAATGA SF1: CCTTCTCGCCATTTGATAGC SR1: GGCGAACCTTCTTACCGTTAT |
mt772, mt853, mt973, mt1283, mt2383 | 1–2,650 |
| apico | F: TTAGTTAATAATCCAGAAACCCATTT | apico15131 | 14,392–15,247 |
| 1 | R*: GTGGATATTCTTTACATACAGA | ||
| F: TGCCTGAGTGGTTAAAAGGAA | apico2122 | 1,473–2,278 | |
| 2 | R*: Ccctgtttacatacaggtg | ||
| F: TCTTTGACCCCCTTGTTTTG | apico20831, apico21188 | 19,599–21,277 | |
| 3 | R*: ctaatgaaggattaacttgtgg | ||
| F*: Gaagctgtacatccttct | apico23803 | 23,595–24,104 | |
| 4 | R: Tatatttaaattacctgattggaa | ||
| F*: AAGTACATCCTTTAATATTTAGAGG | apico4370, apico4878, apico4945, apico5005 | 3,857–5,257 | |
| 5 | R*: CCTATAACTATATCACCATATTTAGG | ||
| F*: GTAGGTACTAAAAGTAATAATTATATATC | apico5715, apico6361, apico6832 | 5,415–7,054 | |
| 6 | R*: TATTATTTTACAATTAAAATAACCTAAAC | ||
| F*: CAACATATAAATTTAGGTACTATAGG | apico9003, apico9096 | 8,785–9,778 | |
| 7 | R: GAGGTTTATATCCAATATTAAAAGG | ||
| 8 | F: CCAAAATTTATAATAAAGGTTCG R: ATCCAACTTCCTATAGGTTTA SF2: ACTAGAAGGGGTAAATGATA Sr2: TATCATTTACCCCTTCTAGT |
apico11066, apico11619, apico11671 | 10,497–12,414 |
| F*: AATTCGATTGGCATTTCACC | apico26659 | 26,347–27,183 | |
| 9 | R: GGATTCATGCTCCGAAGGTA |
F, forward; R, reverse; S, sequencing. F and R pairs were used in PCR amplifications; SF, SR, and primers with
*
labels were used in the sequencing reactions.