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. 2021 Apr 16;9:635815. doi: 10.3389/fped.2021.635815

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Copyright © 2021 Dirks, Fischer, Haase, Holl-Wieden, Hofmann, Girschick and Morbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The extended phenotype of synovial fluid B cells in JIA patients. (A) Hierarchical cluster analysis of mean fluorescence intensities of different markers in CD19⁺ peripheral blood (PB) and synovial fluid (SF) B cells from five JIA patients assessed by flow cytometry. (B) Flow cytometric gating strategy of different SF B cell populations. (C) Distribution of different B cell populations within CD19⁺ PB and SF B cells of five JIA patients. Groups were compared using paired Student's t-test. Bars represent mean frequency with standard deviation and circles individual data points. N, Naïve; NSM, non-switched memory; SM, switched memory, DN, double negative; PC, plasma cells.