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. 2021 Apr 27;16(1):419–430. doi: 10.1515/biol-2021-0044

Figure 2.

Figure 2

Suppressive role of circ_UBR4 depletion in cell cycle progression, proliferation, and migration of ox-LDL-induced human VSMCs. (a) RT-qPCR detected circ_UBR4 and UBR4 expression levels in VSMCs transfected with siRNA targeting circ_UBR4 (si-circ_UBR4), normalized to its negative control (si-NC) transfection. (b–g) VSMCs were co-treated with siRNA transfection for 24 h and ox-LDL (100 μg/mL) treatment for another 24 h. (b) FCM analyzed cell distributions in different cell cycle phases (G0/G1, S, and G2/M) by counting cell number. (c) Colony formation assay measured colony-forming ability by determining number of colonies. (d) CCK-8 assay monitored cell viability by analyzing OD value at 570 nm. (e) Western blotting detected expression of proliferation marker, PCNA. (f) Transwell assay evaluated cell migration ability by counting number of migrated cells. (g) Western blotting detected expression of migration marker, matrix MMP2. *P < 0.05.