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. 2021 Apr 27;16(1):419–430. doi: 10.1515/biol-2021-0044

Figure 3.

Figure 3

Rho/Rho-associated coiled-coil containing kinase 1 (ROCK1) counteracted the role of circ_UBR4 depletion in ox-LDL-induced human VSMCs. (a and b) RT-qPCR and western blotting examined ROCK mRNA expression level and protein expression level in VSMCs transfected with si-NC, si-circ_UBR4, si-circ_UBR4 along with pcDNA plasmid (pcDNA), si-circ_UBR4 along with pcDNA-ROCK1 plasmid (ROCK1). (c–h) VSMCs were co-treated with above transfections for 24 h and ox-LDL (100 μg/mL) treatment for another 24 h. (c) FCM analyzed cell numbers in G0/G1, S, and G2/M phases. (d) Colony formation assay determined number of colonies. (e) CCK-8 assay monitored OD value at 570 nm. (f) Western blotting detected PCNA protein expression level. (g) Transwell assay evaluated number of migrated cells. (h) Western blotting detected MMP2 protein expression level. *P < 0.05.