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. 2021 Apr 16;12:657995. doi: 10.3389/fmicb.2021.657995

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Copyright © 2021 Hicks, Tran-Dao, Mulroney and Bernick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of centrifugation, elevated pH and antibiotic purification methods for L. fusiforms (contaminants shown with arrows). (A) Buoyant cells obtained from a culture of L. fusiformis before any treatment that contained contaminants. Applying only centrifugation resulted in (B) a surface layer of partially purified trichomes and (C) a pellet containing debris and contaminants. (D) Buoyant cells obtained from a culture of L. fusiformis before any treatment. (E) Applying only elevated pH (12.15 pH for 4 days) resulted in a culture with lysed contaminants (likely Microcystis sp. based on morphology; Sant’Anna et al., 2008). (F) The combination of filtration, centrifugation (B), pH treatments (E), and an antibiotic cocktail resulted in an axenic L. fusiformis culture.