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. 2021 Apr 16;12:666233. doi: 10.3389/fimmu.2021.666233

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Copyright © 2021 Elaldi, Hemon, Petti, Cosson, Desrues, Sudaka, Poissonnet, Van Obberghen-Schilling, Pers, Braud, Anjuère and Meghraoui-Kheddar

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IMC staining condition optimization. Two antibody incubation conditions and three antibody dilutions were tested for each antibody of the IMC panel. (A) The markers CD8 and CD206 are representative of the variations induced by incubation time and temperature (1h30 at +4°C and overnight at room temperature (RT)). (B) The markers CD14 and tenascin C are representative of the variations induced by antibody dilution (1/100, 1/200 and 1/400). Scale bar = 100 μm.