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. 2021 Apr 16;12:666233. doi: 10.3389/fimmu.2021.666233

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Copyright © 2021 Elaldi, Hemon, Petti, Cosson, Desrues, Sudaka, Poissonnet, Van Obberghen-Schilling, Pers, Braud, Anjuère and Meghraoui-Kheddar

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization by IMC of structural and cellular TiME components in a single region of cSCC section. Overlaid and single antibody signals representing lymphatic vessels (podoplanin), blood vessels and CAFs (aSMA), nerve fibers (pan-neurofilament), tumor cells (pan-cytokeratin), ECM (fibronectin) and immune cells (CD45) compared to nuclei and HES staining of the same region of cSCC-1 section. Scale bar = 100 μm.