Table 1.

39 markers for tumor microenvironment characterization.

Marker	Supplier	Clone	Metal	In-house coupled	Concentration (μg/ml)	Mix 1	Mix 2
β-Catenin	Abcam	E247	89Y	X	5,0		X
Vimentin	Abcam	EPR3776	139La	X	2,5		X
Tubulin-β-III	Biolegend	TUJ1	141Pr	X	1,3	X
EGFR	Fluidigm	D38b1	142Nd		5,0	X
Ki67	CST	8D5	143Nd	X	2,5		X
CD14	Fluidigm	EPR3653	144Nd		2,5	X
NKp46	Biotechne	195314	145Nd	X	5,0	X
CD16	Fluidigm	EpPR16784	146Nd		5,0		X
CD163	Fluidigm	EDHu-1	147Sm		2,5	X
Pan-cytokeratin	Abcam	AE1-AE3	148Nd	X	1,3	X
CD15	Fluidigm	W6D3	149Sm		1,3	X
PD-L1	Fluidigm	E1L3N	150Nd		5,0		X
NGFR	Biolegend	NGFR5	151Eu	X	2,5	X
CD45	Fluidigm	D9M8I	152Sm		2,5	X
DC-SIGN	Dendritics	102E11-06	153Eu	X	2,5	X
CD11c	Abcam	EP1347Y	154Sm	X	5,0	X
FOXP3	Fluidigm	236A/E7	155Gd		2,5	X
CD4	Fluidigm	EPR6855	156Gd		5,0	X
CD56	Biotechne	123A8	158Gd	X	12,5	X
CD68	Fluidigm	KP1	159Tb		2,5	X
Pan-neurofilament	Biolegend	SMI-312	160Gd	X	1,3		X
CD20	Fluidigm	H1 (FB1)	161Dy		5,0		X
CD8	Fluidigm	D8A8Y	162Dy		1,3	X
Tenascin C	Sigma	BC-24	163Dy	X	2,5	X
CD206	Abcam	Poly	164Dy	X	1,3	X
PD-1	Fluidigm	EPR4877 (2)	165Ho		5,0		X
Langerin	Dendriics	929F3-01	166Er	X	1,3	X
TIM-3	CST	D5D5R	167Er	X	2,5	X
Fibronectin	Abcam	F1	168Er	X	10,0	X
Podoplanin	Biolegend	D2-40	169Tm	X	2,5	X
CD3	Fluidigm	Poly	170Er		2,5	X
CD204	Invitrogen	J5HTR3	171Yb	X	0,6	X
TIGIT	Abcam	BLR047F	172Yb	X	5,0	X
Myeloperoxidase	Biotechne	Poly	173Yb	X	0,6	X
HLA-DR	Fluidigm	YE2/36 HLK	174Yb		1,3	X
Granzyme B	Abcam	EPR20129-217	175Lu	X	1,3		X
DC-LAMP	Dendritics	1010E1.01	176Yb	X	2,5	X
α-SMA	Abcam	EPR5368	194Pt	X	2,5	X
Active-Caspase-3	BD	C92-605	198Pt	X	12,5	X
Cell Intercalator	Fluidigm		191/193Ir		125 (μM)