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. 2021 Apr 16;12:646307. doi: 10.3389/fphar.2021.646307

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Copyright © 2021 Xu, Ge, Hu, Yu, Guo and Miao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sevoflurane postconditioning attenuates oxidant stress and inflammatory response. Mice were subjected to ischemia, then post-treatment with inhalation of 2% Sevo for 2 h immediately at the onset of reperfusion. (A, B) The contents of MDA and SOD were measured by ELISA assays (n = 6/group). (C) The expressions of GPx and p22phox were detected by Western Blot (n = 6/group). (D–G) The inflammatory cytokines including TNF-α, IL-1β, IL-6, and IL-10, were evaluated by ELISA assays (n = 6/group). (H) The protein expressions of TNF-α, IL-1β, IL-6, and IL-10 were determined by Western Blot (n = 6/group). (I) Animal survival was recorded at the indicated time points (n = 10/group). All data were measurement data which were expressed as mean ± standard deviation and analyzed by one-way analysis of variance. *p < 0.05, **p < 0.01 vs. Sham group; ^## p < 0.01 vs. HIRI group.