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. 2021 Apr 16;12:646307. doi: 10.3389/fphar.2021.646307

FIGURE 5.

FIGURE 5

Sevoflurane postconditioning attenuates oxidant stress and inflammatory response through regulation of miR-142. The agomiR-142 and antagomiR-142 were injected into mice, and then the mice were subjected to ischemia, post-treatment with inhalation of 2% Sevo for 2 h immediately at the onset of reperfusion. (A, B) The contents of MDA and SOD were measured by ELISA assays (n = 6/group). (C) The expressions of GPx and p22phox were detected by Western Blot (n = 6/group). (D–G) The inflammatory cytokines including TNF-α, IL-1β, IL-6 and IL-10, were evaluated by ELISA assays (n = 6/group). (H) The protein expressions of TNF-α, IL-1β, IL-6, and IL-10 were determined by Western Blot (n = 6/group). (I) Animal survival was recorded at the indicated time points (n = 10/group). All data were measurement data which were expressed as mean ± standard deviation and analyzed by one-way analysis of variance. *p < 0.05, **p < 0.01 vs. HIRI group; ## p < 0.01 vs. HIRI + Sevo group.