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. 2021 Apr 16;12:639716. doi: 10.3389/fphar.2021.639716

FIGURE 2.

FIGURE 2

Effects of different PMA concentrations on THP-1 post-differentiation cytokine release. (A) IL-8 and (B) TNFα release by THP-1 cells differentiated for 65 h with increasing concentrations of PMA (10, 20, and 40 ng/ml) and rested for 24 or 48 h after PMA removal. (C) IL-8 and (D) TNFα release by THP-1 cells differentiated with increasing concentrations of PMA (10, 20, and 40 ng/ml) for 65 h, rested for 24 or 48 h after PMA removal, and treated with 10 ng/ml LPS for 24 h. Data are presented as the average of three technical replicates with corresponding standard deviation. Statistical differences were calculated between the 65-h induced sample and the rested samples (A,B) or between LPS-treated and untreated cultures (C,D) by means of two-tailed t-tests. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ns, p > 0.05 (A,B). Equivalence comparisons and linear regression analysis are provided in Supplementary Tables S3 and S4. R24h = cells rested for 24 h; R48h = cells rested for 48 h. Abbreviations: PMA, phorbol 12-myristate.