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. 2021 Apr 16;11:656628. doi: 10.3389/fonc.2021.656628

Figure 2.

Figure 2

Targeting FOSL1 suppresses miR-21-5p expression by interacting with the MIR21-SE. (A) ChIP-seq data revealed SE was formed around the MIR21 gene region and knockdown of FOSL1 suppressed the enrichment of MED1 and FOSL1 in the MIR21-SE. (B, C) Disruption of SE by JQ1 and iBET-151 inhibited the expression of miR-21-5p. **P< 0.01, ***P < 0.001 by one-way ANOVA. (D–F) The enrichments of MED1, BRD4 and FOSL1 in the MIR21-SE were eliminated in HNSCC cells upon JQ1 and iBET-151 treatment. **P < 0.01 by one-way ANOVA. (G–I) The enrichments of MED1 and FOSL1 in the MIR21-SE were eliminated in HNSCC cells treated with FOSL1 siRNA. **P < 0.01 by one-way ANOVA. (J) Knockdown of FOSL1 significantly suppressed the MIR21-SE luciferase activity. ***P < 0.001, **P<0.01 by one-way ANOVA. (K) MIR21-SE fragment was capable of elevating the luciferase reporter activity as compared to the negative control. **P < 0.01 by Student’s t-test (L, M) Knockdown of FOSL1 inhibited the expression of miR-21-5p. **P < 0.01, ***P < 0.001 by one-way ANOVA.