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. 2021 Apr 16;12:618561. doi: 10.3389/fimmu.2021.618561

Figure 1.

Figure 1

Characterization of mice with Myeloid-specific deletion of Prak. (A) Peritoneal macrophages (PM) and total splenocytes were collected from Prak knockout (KO, LysM-Cre Prak flox/flox) and wild type (Prak flox/flox) mice. The expression of PRAK protein was detected by Western blotting. (B) Total cells in the peritoneal lavage from untreated and thioglycollate (TG)-treated mice were stained for CD11b and F4/80 expression. Representative dot plots are shown on the left and the percentage of CD11b+ F4/80+ macrophages are presented as mean ± SD on the right (n=4 for each group). (C) Surface expression of MHC II, CD80, CD86, CD40 and CD14 in Prak-deficient and wild type peritoneal macrophages was analyzed by flow cytometry. Representative histograms from three independent experiments are shown in the top panel and the mean fluorescence Intensity is presented as mean ± SD at the bottom. *P<0.05 by t-test. ns, not significant.