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. 2021 Apr 16;12:640297. doi: 10.3389/fphar.2021.640297

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Copyright © 2021 Zhang, Jiang, Wang, Lin, Zhang, Zhang, Zhu, Ng, Qu, Sze and Yung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AGNHW pre-treatment restored neuronal loss in MCAO model (A,B) Representative images (A) and statistical analysis results (B) of Nissl staining in the infarct area of the ischemic cortex in MCAO rats with or without pre-treatment with AGNHW (C,D) Representative images (C) and statistical analysis results (D) of immunofluorescent staining to visualize NeuN in the infarct area of the ischemic cortex in MCAO rats with or without pre-treatment with AGNHW (E,F) Representative images (E) and statistical analysis results (F) of immunofluorescent staining to visualize DCX in the infarct area of the ischemic cortex in MCAO rats with or without pre-treatment with AGNHW. N = 5 rats per group. Data are means ± S.D. ^## p < 0.01, compared with the sham group; **p < 0.01, compared with the vehicle group; ^aa P<0.01, compared with AGNHW-L group; ^b P<0.05 and ^bb P<0.01, compared with the AGNHW-M group.