Table 2.
TDP1 Inhibition of the Active Compounds
| IC50 (μM)a |
||
|---|---|---|
| compd | fluorescence assay | gel-based assay |
| 39a | 24 ± 0.80 | 16 ± 0.40 |
| 39b | 18 ± 1.7 | 13 ± 4.0 |
| 40a | 58 ± 20 | 40 ± 14 |
| 40b | 15 ± 2.7 | 27 ± 5.2 |
| 41a | 7.0 ± 1.4 | 8.2 ± 1.3 |
| 41b | 20 ± 1.7 | 21 ± 1.2 |
| 42b | 19 ± 4.8 | 20 ± 5.4 |
IC50 values were defined as the concentration of compound that inhibits 50% of enzyme activity. Every experiment was repeated at least three times independently.