. Author manuscript; available in PMC: 2021 Apr 30.

Published in final edited form as: J Med Chem. 2018 Oct 31;61(22):9908–9930. doi: 10.1021/acs.jmedchem.8b00639

Table 2.

TDP1 Inhibition of the Active Compounds

	IC₅₀ (μM)^a
compd	fluorescence assay	gel-based assay
39a	24 ± 0.80	16 ± 0.40
39b	18 ± 1.7	13 ± 4.0
40a	58 ± 20	40 ± 14
40b	15 ± 2.7	27 ± 5.2
41a	7.0 ± 1.4	8.2 ± 1.3
41b	20 ± 1.7	21 ± 1.2
42b	19 ± 4.8	20 ± 5.4

IC₅₀ values were defined as the concentration of compound that inhibits 50% of enzyme activity. Every experiment was repeated at least three times independently.