Fig. 3.

NaBC1/α_5, α_v integrins co-localization. a) Images of nuclei (blue) and co-localization dots (magenta) of C2C12 cultured for 0.5 h onto fibronectin-coated glass substrates, serum depleted and borax (Glass-B) in the culture medium. Scale bar represents 10 μm. b) Image analysis quantification of the number of co-localization dots of NaBC1/α₅ and NaBC1/α_v. Co-localization levels increased after NaBC1 activation with borax. n = 20 images/condition from 3 different biological replicas. Statistics are shown as mean ± standard deviation. Data were analyzed by an unpaired t-test applying Welch's corrections (P = 0.05). *p < 0.05, ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)