Fig. 4.

Characterization of borax effects in extracellular matrix (fibronectin) reorganization and C2C12 cell migration. a) Fibronectin (FN) reorganization by C2C12 cells after 3 h of culture in the presence of serum and borax (Glass-B) in the culture medium. Immunofluorescence detection of fibronectin (magenta), actin cytoskeleton (green) and nuclei counterstained with DAPI (blue). Dark area around each cell was quantified to establish the reorganization area. Scale bar represents 50 μm. n = 10 images from 3 different biological replicas for each condition. b) Phase contrast images of C2C12 representing the position of cells after 0 h, 12 h and 24 h. Red arrows indicate the position of a selected migrating cell over time in the different conditions. Quantification of speed average and total distance covered by tracked cells from time-lapse microscopy experiments (see supplementary videos). n = 6 different tracked cells per condition and 139 measurements per cell for a total of 24 h. Statistics are shown as mean ± standard deviation. Data were analyzed by an unpaired t-test applying Welch's corrections (P = 0.05). *p < 0.05, ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)