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. 2021 Apr;123:112003. doi: 10.1016/j.msec.2021.112003

Fig. 7.

Fig. 7

Evaluation of the borax effects in skeletal muscle regeneration in a mouse model of acute injury with cardiotoxin (CTX). a) Analysis of tibialis anterior (TA) muscle regeneration 15 days after injury by Hematoxylin & Eosin staining. Images show representative regenerating myofibers with centrally located nuclei (ALG and ALG-B). Tibialis anterior control represents healthy tissue without induced muscle injury. Note the myofibers with periphery located nuclei. Scale bar 200 μm. Image analysis quantification of regenerating myofibers in the different conditions. Active-NaBC1 significantly increases regenerating fibers characterized by the presence of centrally located nuclei. n > 70 images from 6 different mice per condition. Image analysis quantification for morphological evaluation of the regenerating myofibers. Active-NaBC1 significantly increases myofiber cross-sectional area (CSA) associated with an accelerated regenerative response. n > 700 fibers from 6 different mice per condition. Statistics are shown as mean ± standard deviation. Data were analyzed by an ordinary one-way ANOVA test and corrected for multiple comparisons using Tukey's correction analysis (P = 0.05). Statistics indicate differences between groups. ****p < 0.0001. b) Immunohistochemically stained tibialis anterior sections for evaluation of different markers related to muscle regeneration using Chloride channel (CLC-1 - magenta), laminin (LM - yellow), CD31 (red) and fibronectin that raises after an injury (FN – green) counterstained with DAPI (blue) for nuclei visualization. Image analysis quantification of the integrated density as a measure of the total detected fluorescence. Active-NaBC1 promotes higher expression of CLC-1, laminin and CD31 indicative of an accelerated regenerative response. Scale bar 100 μm. n = 10 images from 3 different mice per condition. Statistics are shown as mean ± standard deviation. Data were analyzed by an ordinary one-way ANOVA test and corrected for multiple comparisons using Tukey's correction analysis (P = 0.05). ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)