Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 28;220(7):e202009179. doi: 10.1083/jcb.202009179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Venkatesan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Large C-domain truncations or combined N-domain and C-domain dimer interface mutations are required to abrogate CRT_Del52-mediated cell proliferation. (A–D) Cytokine-independent proliferation of Ba/F3-Mpl cells expressing full-length untagged CRT_WT, CRT_Del52, indicated CRT_Del52 mutants, or control cells (Vec). Data are averaged from three separate viral transductions of Ba/F3-Mpl cells and a total of 5 independent proliferation experiments (A), eight separate viral transductions of Ba/F3-Mpl cells and a total of 10–13 independent experiments (B), two to three separate viral transductions of Ba/F3-Mpl cells and a total of 3–6 experiments (C), or two separate retroviral transductions of Ba/F3-Mpl cells and a total of 5 independent experiments (D). Mean ± SEM is shown, with statistical significance assessed via ordinary one-way ANOVA from the indicated days of proliferation assay. Statistically significant means are indicated as *, P < 0.05; **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001. (E–G) Lysates from Ba/F3-Mpl cells expressing indicated constructs or control cells expressing Mpl alone (Vec) were directly loaded for immunoblotting analyses (labeled as lysates) or immunoprecipitated with anti-CRT(C_mut) antibody (for CRT_Del52 and its variants) or with anti-CRT(Thermo) antibody (for CRT_WT), and subsequent immunoblotting was undertaken with the indicated antibodies. Results are representative of three (E and F) or four (G) independent experiments. Nonspecific interactions in the absence of primary antibody are shown by the lysate + beads lanes. Ab, antibody; MW, molecular weight.