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. 2021 Apr 28;220(7):e202009179. doi: 10.1083/jcb.202009179

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© 2021 Venkatesan et al.

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Figure S2. — Mutant CRT preferentially interacts with Mpl in HEK293T cells and MPN patient platelets. (A) Lysates from HEK293T cells expressing full-length untagged WT or mutant CRTs (Ins5 or Del52) and Mpl or control cells expressing Mpl alone (Vec) were immunoprecipitated with anti-Mpl antibody, and subsequent immunoblotting was undertaken with the indicated antibodies. Data shown are representative of two independent experiments. (B and C) Lysates from indicated healthy donor (HC) or MPN patient platelets (Del52) were immunoprecipitated with anti-Mpl antibody (B) or anti-CRT(C_mut) antibody (C) and subsequently analyzed by immunoblotting with the indicated antibodies. CRT mutant patients are indicated as Del52. In all panels, nonspecific interactions in the absence of primary antibody are shown by the lysate + beads lanes. Ab, antibody; MW, molecular weight.