Figure 1.

Decursin inhibits GC cell proliferation. A. SNU216 and NCI-N87 cells were treated with indicated concentrations of decursin for indicated times. B, C. After decursin treatment for 24 h, cell growth was reduced, as demonstrated by bright field imaging and clonogenic assay (×100). Scale bar: 50 μm. D. Incorporated EdU was stained with anti-EdU, and cell nuclei were counterstained with Hoechst 33342 (×400). Scale bar: 50 μm. E. Representative results of cell cycle analysis by flow cytometry. F. Confirmation of expression of several cell cycle-related proteins. Full-length blots are presented in Supplementary Figure 6. n.s.: not significant; *P < 0.05; **P < 0.01; ***P < 0.001.