Figure 5.

Cathepsin C regulates cell cycle through E2F3. A, B. Cell proliferation and cell cycle assay after treatment with siCTSC. Both cell lines were transfected with siRNA targeting CTSC. After 24 h, cells were dissociated and seeded into 96-well plates. Proliferation was measured by CCK-8 assay at indicated times. Cell cycle analysis by FACS was performed 48 h after transfection. C. E2F3 mRNA and protein levels after treatment with siCTSC were confirmed by RT-PCR and western blotting. Full-length blots are presented in Supplementary Figure 6. D. Treatment with decursin induced reduction of E2F3 expression in a dose-dependent manner. Full-length blots are presented in Supplementary Figure 6. E. Following transfection of siE2F3, expression levels of each protein were confirmed by western blotting. Full-length blots are presented in Supplementary Figure 6. F. After 24 h of siE2F3 transfection, cells were dissociated and re-seeded in 96-well plates; CCK-8 assays were then performed at indicated times. G. E2F3 knockdown induced G0/G1 cell cycle arrest. n.s.: not significant; *P < 0.05; **P < 0.01; ***P < 0.001.