Figure 2.

PNN promotes PCa cell proliferation in vitro. A. PNN protein levels in prostate cancer cells. Western blotting was performed to detect PNN expression, GAPDH was measured as control. B. RT-qPCR was performed to analyse PNN mRNA expression in prostate cancer cells. GAPDH was measured as control. The fold changes were calculated by relative quantification (2^-ΔΔCt). C. Verification of PNN expression by western blotting after prostate cancer cells infecting PNN-shRNA-lentivirus or transfecting with PNN over-expression vector. D. Down-regulation of PNN repressed cell growth in PC-3 cells. Cell growth was detected by MTS assay. E. PNN up-regulation showed proliferation-promoting effect in DU145 cells. F. The tumorigenesis ability was reduced in PNN-silenced PC-3 cells. PC-3 cell tumorigenesis was detected by colony formation assays with incubation for 14 days followed by crystal violet staining. *P < 0.05, **P < 0.01, ***P < 0.001.