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. 2021 Apr 15;11(4):1369–1390.

Figure 4.

Figure 4

LHPP down-regulated the protein level and enzymatic activity of PKM2. A. Venn diagram of Flag-LHPP interacting proteins in LHPP-overexpression U87MG and U118MG cells, and the coincident proteins anti-Flag antibody was used to pull down the Flag-LHPP complex. B. Top 10 KEGG pathways of coincident Flag-LHPP interacting proteins in LHPP-overexpression U87MG and U118MG cells. C. Protein-protein interaction network of coincident Flag-LHPP interacting proteins in LHPP-overexpression U87MG and U118MG cells. D. Top 10 Flag-LHPP interacting proteins in U87MG and U118MG cells. E. Western blot assay for protein expression level of PKM2 in U87MG and U118MG cells after LHPP plasmids or empty vector were transfected. F. Western blot detect the protein expression level of monomeric, dimeric, and tetrameric PKM2 in U87MG and U118MG cells after LHPP plasmids or empty vector were transfected. G. Statistical analysis of relative protein expression level of dimeric and tetrameric PKM2 in U87MG and U118MG cells after LHPP plasmids or empty vector were transfected (n=3). H. Immunohistochemical staining of LHPP and PKM2 in paraffin sections of the brain of the tumor-burdened mice (×400). I. Pyruvate kinase activity (PK) was detected in U87MG and U118MG cells after LHPP plasmids or empty vector were transfected, and the statistical analysis of relative PK (n=4). The data were expressed as the mean ± SEM (*P<0.05, **P<0.001, ***P<0.0001).