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LH inhibits the mobility of melanoma cells. A. The wounds on cell layers under the incubation of 20 μM LH or DMSO were observed at different times (0, 24, 48, 72 h) and the closure rates were calculated. Scale bar, 100 μm. B, C. Migration and invasion of the cells under LH (20 μM) or DMSO treatment. Scale bar, 100 μm. D, E. The protein levels of E-cadherin, N-cadherin and Vimentin in LH treated cells were detected by western blotting. Mean ± SD; **P<0.01, ***P<0.001.
