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. 2021 Apr 15;11(4):1391–1409.

Figure 5.

Figure 5

Overexpressing p21 attenuates the anti-proliferative effect of LH. A. Viability of p21/vector-overexpressing A375 and MV3 cells under the treatment of 20 μM LH was measured by MTT assay. B. EdU staining of the p21/vector-overexpressing cells after LH (20 μM) treatment for 48 h. Scale bar, 50 μm. C. Cell cycle distribution of the p21/vector-overexpressing cells after LH (20 μM) treatment for 48 h. D, E. After being treated with LH (20 μM) or DMSO for 48 h, the protein levels of p21, CDK1, CDK2 and Cyclin E1 in p21/vector-overexpressing cells were detected by western blotting. Mean ± SD; *P<0.05, **P<0.01, ***P<0.001.