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. 2021 Apr 15;11(4):1347–1368.

Figure 1.

Figure 1

BLM was highly expression in PRAD than normal cells, Co-IP and GST pull down were carried out, IF results shows that the BLM and EZH2 proteins are co-located in PC3. A. Western blot analysis of BLM expression in human normal prostate (RWPE-1) and PRAD cell lines (PC3, LNCap, 22RV1). B. BLM interaction proteins were obtained by Co-IP test under physiological conditions, IP and IgG control group were send company for MS identification. C. BLM and EZH2 mRNA levels in the PRAD TCGA dataset (normal vs. primary tumor). D. The relationship between Gleason score and mRNA expression level of BLM and EZH2 in the PRAD TCGA dataset. E. EZH2 is significantly positively correlated with BLM mRNA level in the PRAD TCGA dataset. F. Protein-protein interaction network of BLM and EZH2, the protein network was predicted using STRING database. G, H. The interaction between BLM and EZH2 was confirmed by Co-IP and GST-pull down. I. The localization of BLM and EZH2 proteins in PC3, LNCap and 22RV1 cells were detected by indirect immunofluorescence assay.