Skip to main content
. 2021 Apr 15;11(4):1347–1368.

Figure 4.

Figure 4

The expression of P53 was upregulated after BLM and EZH2 knockdown, and BLM may affect the P53 signaling pathway by interacting with EZH2 protein. (A) PC3 cells were transfected by siNC, siBLM, siEZH2-1 and siEZH2-2 for 48 h, and then the expressions of BLM, EZH2 and P53 in PC3 cells were detected by Western blot, GAPDH is used as a loading control. (B) PC3 cells were treated as described in (A), and then the proliferation ability was detected after 24 h, 48 h and 72 h. Data represented one of six independent experiments. (C-E) PC3 cells were treated as described in (A), and the migration, invasion, clone formation and scratch-wound were identified. Columns, means of three independent experiments; bars, SD (n=3). *, P<0.05.