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. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Environ Res. 2018 Feb 22;164:45–52. doi: 10.1016/j.envres.2018.02.011

Figure 2: Maternal BPA Effects on Offspring NPC Differentiation and Neuropeptides.

Figure 2:

Hypothalamic NPCs from Control and BPA 1 day old male newborns were cultured in differentiation media. (A) Double immunostained images of (x40; scale bar = 50μm) of DAPI (blue, nuclear stain) and Ngn3 (red, proneurogenic factor). (B) Protein expression of Ngn3. (C) Protein expression of appetite (AgRP) and satiety (POMC) neuropeptides. Values are fold change (mean±SE) of n=6 of pooled hypothalami from each of 6 litters per group. * P< 0.05 BPA (□) vs. Control (■).