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. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Environ Res. 2018 Feb 22;164:45–52. doi: 10.1016/j.envres.2018.02.011

Figure 3: In Vitro BPA Effects on NPC Proliferation.

Figure 3:

Hypothalamic NPCs from 1 day old Control newborns were cultured in complete media and treated with DMSO (Control) or BPA (10 μM unless otherwise specified) for 5 days. (A) Images of NPCs (x20; scale bar = 50μm) and proliferative index measured at 570 OD of NPCs treated with BPA (1, 10, 20 μM). (B) Protein expression of Nestin (NPC marker) and bHLH proliferative (Hes1). (C) Immunostained images of (x40) Nestin (red), Hes1 (green) and DAPI (blue, nuclear stain). Values are fold change (mean±SE) of n=4 of pooled NPCs from each of 4 litters. * P< 0.05 BPA (□) vs. Control (■).