FIGURE 3.

TAB182 affects radioresistance in radioresistant TE‐1R cells. (A) Scheme of radioresistant TE‐1R cells generation. (B) Western blot confirmed TAB182 upregulation in TE‐1R cells compared with parent TE‐1 cells and TAB182 expression was successfully downregulated by shRNA. (C) Clonogenic survival assay of TE‐1, TE‐1R, and TAB182 knockdown TE‐1R cells after ionizing radiation (n = 3). (D) Parameters of multitarget click model analysis after IR. (E) Immunofluorescent staining of γ‐H2A.X foci in the control group and TAB182 knockdown group at different time points after 4 Gy IR. *p < 0.05, ***p < 0.001 versus parent TE‐1 cells. ^# p < 0.05 versus control TE‐1R cells